leptin receptor mrna expression in taleshi ram gonads

Authors

m. roostaei-ali mehr

r. rajabi-toustani

r. motamedi-mojdehi

abstract

leptin is secreted mainly by fat, which is involved in energy metabolism and reproduction. leptin and itsreceptor (ob-r) have been detected in human spermatozoa and testis, thus it can be concluded leptin involves in male physiology. the goal of this study was determination of the presence of leptin receptor mrna in the ram testis, epididymis and spermatozoa by reverse transcription polymerase chain reaction (rt-pcr). ejaculated spermatozoa from ten fertile taleshi ram were collected by means of an artificial vagina. testes, placental cotyledons and adrenal glands were obtained from abattoir. placental cotyledons and adrenal glands were used as the positive control. epididymal spermatozoa recovery was performed from epididymis. to purify spermatozoa, motile sperm were isolated by the swim-up procedure. total rna was isolated from epididymal spermatozoa, ejaculated spermatozoa, adrenal gland and placental cotyledon and then they were purified. the mrna for the long form (ob-rb) and the short form (ob-ra) of leptin receptor was detected in testis. rt-pcr analysis of total rna from epididymal spermatozoa and ejaculated spermatozoa revealed the presence of leptin mrna in these cells. the mrna for ob-rb was observed in epididymis and epididymal spermatozoa, but the ob-ra mrna was absent. the presence of ob-ra mrna was found in ejaculated spermatozoa, whereas ob-rb mrna did not exist. it can be concluded that the mrna for leptin receptor is present in ram gonads and spermatozoa.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Leptin receptor mRNA expression in Taleshi ram gonads

Leptin is secreted mainly by fat, which is involved in energy metabolism and reproduction. Leptin and itsreceptor (Ob-R) have been detected in human spermatozoa and testis, thus it can be concluded leptin involves in male physiology. The goal of this study was determination of the presence of leptin receptor mRNA in the ram testis, epididymis and spermatozoa by reverse transcription polymerase ...

full text

P-69: Expression of Leptin Receptor mRNA in Ovine Corpus Luteum

Background: Many hormones are involved in the regulation of reproduction. Leptin hormone which is mainly secreted by adipose tissue plays an important role in energy homeostasis and reproduction. It seems that leptin is an important linkage between body metabolism and reproductive system. Moreover, it has been shown that leptin and leptin receptor express in reproductive organs of some species....

full text

Leptin Receptor in Ram Epididymal Spermatozoa

This experiment was designed to investigate leptin receptors (Ob-R and Ob-Rb) mRNA in ram epididymal spermatozoa by RT-PCR. Ten testes were obtained from abattoir and epididymal spermatozoa recovery was performed. To purify spermatozoa, motile sperm were isolated by the swim-up procedure. Total RNA was isolated from epididymal spermatozoa and placental cotyledon, as a positive control, and then...

full text

Expression of leptin and leptin receptor transcript in ovine corpus luteum

Background: Leptin, the product of the obesity (ob) gene, acts as a signaling adipokine for modulating food intake, energy metabolism and reproductive functions in mammals. Leptin’s effects on the reproductive system at various levels of the hypothalamic-pituitary-gonadal axis have been established. Moreover, the direct and local effect of leptin on bovine oocyte maturation and corpus luteum fu...

full text

My Resources

Save resource for easier access later


Journal title:
iranian journal of veterinary research

Publisher: shiraz university

ISSN 1728-1997

volume 14

issue 4 2013

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023